heat labile udg enzyme (New England Biolabs)

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New England Biolabs heat labile udg enzyme
Heat Labile Udg Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2304 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/udg/Uracil+DNA+Glycosylase/pmc13090509-37-3-6
Average 99 stars, based on 2304 article reviews

heat labile udg enzyme - by Bioz Stars, 2026-09

99/100 stars

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Amplification:

Article Title: Scalable single-cell total RNA sequencing unifies coding and noncoding transcriptomics.
Article Snippet: .. Nature Biotechnology Article https://doi.org/10.1038/s41587-026-03068-6 cDNA amplification and rRNA depletion Following reverse transcription, emulsion droplets were broken according to 10x recovery steps. cDNA was then treated with UDG (NEB, M0280S) to remove dUTSO fragments at 37 °C for 50–60 min. ..

Article Title: MED12 Dictates Epithelial Ovarian Cancer Cell Ferroptosis Sensitivity via YAP-TEAD1 Signaling.
Article Snippet: .. The Ulabeled strand was digested with UDG (NEB, Ipswich, MA, USA), and the adapter-ligated fragments were amplified by PCR under the following conditions: 95 ◦C for 3 min; 8 cycles of 98 ◦C for 15 s, 60 ◦C for 15 s, 72 ◦C for 30 s; final extension at 72 ◦C for 5 min. .. The resulting cDNA libraries had an average insert size of 300 ± 50 bp and were subjected to 2 × 150 bp paired-end sequencing on an Illumina NovaSeq 6000 platform (LC-Bio) (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions.

Article Title: Low-Intensity Focused Ultrasound Enhances Meningeal Lymphatic Drainage for Preventing Cognitive Decline in Alzheimer’s Disease
Article Snippet: .. Uracil residues were removed by UDG (NEB #M0280) treatment before PCR amplification. ..

Reverse Transcription:

Emulsion:

Quantitative RT-PCR:

Article Title: The actin motor protein MYO10 facilitates post-entry spread of respiratory syncytial virus
Article Snippet: .. RSV genome copy numbers were quantified using the Luna® Probe One-Step RT-qPCR 4× Mix with UDG (New England Biolabs; M3029) following the manufacturer’s protocol, with an RSV-specific probe. ..

Adapter Ligation:

Article Title: Harnessing piezoelectric stimulation to modulate PI3K-AKT signaling for intervertebral disc regeneration
Article Snippet: .. Following adapter ligation, cDNA fragments were size-selected for an average insert size of 300 bp ± 50 bp using magnetic beads, and the second strand was degraded with UDG (NEB). ..

Magnetic Beads:

Polymerase Chain Reaction:

Article Title: Astragaloside IV targets TUBB4B to inhibit proliferation and promote apoptosis of pituitary tumor cells via the STMN1/ERK pathway
Article Snippet: Then, E. coli DNA polymerase I (NEB, cat. no. m0209) and RNase H (NEB, cat. no. m0297) were used for double-strand synthesis to convert the composite double-stranded RNA-DNA hybrid into double-stranded DNA. .. The double-stranded DNA was digested with UDG (NEB, cat. no. m0280), followed by PCR (pre-denaturation at 95°C for 3 min; 8 cycles of denaturation at 98°C for 15 sec each; annealing at 60°C for 15 sec; extension at 72°C for 30 sec, followed by a final extension at 72°C for 5 min) to generate a library consisting of fragments ranging from 300±50 bp. .. Finally, the library was sequenced using Illumina NovaseqTM 6000 (LC Bio Technology Co., Ltd.) in a paired-end (PE150) sequencing mode.

Article Title: Astragaloside IV targets TUBB4B to inhibit proliferation and promote apoptosis of pituitary tumor cells via the STMN1/ERK pathway.
Article Snippet: Then, E. coli DNA polymerase I (NEB, cat. no. m0209) and RNase H (NEB, cat. no. m0297) were used for double‐strand synthesis to convert the composite double‐stranded RNA‐DNA hybrid into double‐stranded DNA. .. The double‐stranded DNA was digested with UDG (NEB, cat. no. m0280), followed by PCR (pre‐denaturation at 95 ̊C for 3 min; 8 cycles of denaturation at 98 ̊C for 15 sec each; annealing at 60 ̊C for 15 sec; extension at 72 ̊C for 30 sec, followed by a final extension at 72 ̊C for 5 min) to generate a library consisting of fragments ranging from 300±50 bp. .. Finally, the library was sequenced using Illumina NovaseqTM 6000 (LC Bio Technology Co., Ltd.) in a paired‐end (PE150) sequencing mode.

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